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  2. Live-cell imaging - Wikipedia

    en.wikipedia.org/wiki/Live-cell_imaging

    This opens up the future possibility of 3-dimensional live-cell imaging by means of fluorescence techniques. [22] Quantitative phase-contrast microscopy with rotational scanning allow 3D time-lapse images of living cells to be acquired at high resolution. [23] [24] [4]

  3. Lattice light-sheet microscopy - Wikipedia

    en.wikipedia.org/wiki/Lattice_light-sheet_microscopy

    Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...

  4. IMOD (software) - Wikipedia

    en.wikipedia.org/wiki/IMOD_(software)

    IMOD will also open TIF files and includes a set of programs to convert between image formats including common microscopy formats like ".raw" and ".dm4". Vector Format : IMOD saves and opens vector data in the form of contour (polygons) and meshes in an IMOD binary file format , typically with a ".mod" or ".fid" extension.

  5. Transmission electron cryomicroscopy - Wikipedia

    en.wikipedia.org/wiki/Transmission_electron_cryo...

    CryoTEM image of GroEL suspended in amorphous ice at 50 000 × magnification Structure of Alcohol oxidase from Pichia pastoris by CryoTEM. Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid ...

  6. Multifocal plane microscopy - Wikipedia

    en.wikipedia.org/wiki/Multifocal_plane_microscopy

    The schematic of a multifocal plane microscope. Multifocal plane microscopy (MUM), also known as multiplane microscopy or multifocus microscopy, is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.

  7. Amira (software) - Wikipedia

    en.wikipedia.org/wiki/Amira_(Software)

    Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.

  8. Microscope image processing - Wikipedia

    en.wikipedia.org/wiki/Microscope_image_processing

    Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine, biological research, cancer research, drug testing, metallurgy, etc. A number of ...

  9. 3D cell culture - Wikipedia

    en.wikipedia.org/wiki/3D_cell_culture

    A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]

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