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2. Live-cell imaging - Wikipedia

   en.wikipedia.org/wiki/Live-cell_imaging

   Live-cell imaging is the study of living cells using time-lapse microscopy. It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics. [1] Live-cell imaging was pioneered in the first decade of the 21st century.

3. Live single-cell imaging - Wikipedia

   en.wikipedia.org/wiki/Live_single-cell_imaging

   In systems biology, live single-cell imaging is a live-cell imaging technique that combines traditional live-cell imaging and time-lapse microscopy techniques with automated cell tracking and feature extraction, drawing many techniques from high-content screening. It is used to study signalling dynamics and behaviour in populations of ...

4. Time-lapse microscopy - Wikipedia

   en.wikipedia.org/wiki/Time-lapse_microscopy

   This is known as live-cell imaging. A few tools have been developed to identify and analyze single cells during live-cell imaging. [2] [3] [4] Time-lapse microscopy is the method that extends live-cell imaging from a single observation in time to the observation of cellular dynamics over long periods of time.

5. Two-photon excitation microscopy - Wikipedia

   en.wikipedia.org/wiki/Two-photon_excitation...

   Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.

6. Photoactivated localization microscopy - Wikipedia

   en.wikipedia.org/wiki/Photoactivated...

   A growing number of dyes are used for PALM, STORM and related techniques, both organic fluorophores and fluorescent proteins. Some are compatible with live cell imaging, others allow faster acquisition or denser labeling. The choice of a particular fluorophore ultimately depends on the application and on its underlying photophysical properties. [5]

7. Multifocal plane microscopy - Wikipedia

   en.wikipedia.org/wiki/Multifocal_plane_microscopy

   The schematic of a multifocal plane microscope. Multifocal plane microscopy (MUM), also known as multiplane microscopy or multifocus microscopy, is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen.