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Lugol's iodine is a liquid made up of two parts potassium iodide for every one part elemental iodine in water. [8] Lugol's iodine was first made in 1829 by the French physician Jean Lugol. [7] [8] It is on the World Health Organization's List of Essential Medicines. [9] [10] Lugol's iodine is available as a generic medication and over the ...
Lugol's iodine solution is always added after addition of crystal violet to form a stable complex with crystal violet that strengthen the bonds of the stain with the cell wall. [ 4 ] Gram staining is almost always the first step in the identification of a bacterial group.
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Melzer's reagent is an aqueous solution of chloral hydrate, potassium iodide, and iodine.Depending on the formulation, it consists of approximately 2.50-3.75% potassium iodide and 0.75–1.25% iodine, with the remainder of the solution being 50% water and 50% chloral hydrate.
The image on the right stains with Lugol's iodine, and identifies a squamous cell carcinoma, which does not pick up the stain. Chromoendoscopy is a medical procedure wherein dyes (often the same stains used in histology ) are instilled into the gastrointestinal tract at the time of visualization with fibre-optic endoscopy .
The "2% free iodine" description is based on the quantity of elemental iodine, not sodium/potassium iodide. [citation needed] USP Strong Iodine Tincture is defined in the NF as containing in each 100 mL, 6.8 to 7.5 gram of iodine, and 4.7 to 5.5 gram of potassium iodide. Purified water is 50 mL and the balance is alcohol.