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This reaction takes place at 25 °C. In the second step, reverse transcriptase synthesis extends the DNA oligonucleotides that have annealed to the mRNA’s extended 3’ end. In order to ensure that DNA oligomers hybridised to internal poly(A) sequences do not serve as primers for reverse transcription, the second step is carried out at 55 °C.
Polyadenylation is the addition of a poly(A) tail to an RNA transcript, typically a messenger RNA (mRNA). The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature mRNA for translation.
The proximity extension assay (PEA) is a method for detecting and quantifying the amount of many specific proteins present in a biological sample such as serum or plasma. [1] The method is used in the research field of proteomics , specifically affinity proteomics, [ 2 ] where in one searches for differences in the abundance of many specific ...
In field theory, a branch of mathematics, the minimal polynomial of an element α of an extension field of a field is, roughly speaking, the polynomial of lowest degree having coefficients in the smaller field, such that α is a root of the polynomial. If the minimal polynomial of α exists, it is unique. The coefficient of the highest-degree ...
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Separable polynomials are used to define separable extensions: A field extension K ⊂ L is a separable extension if and only if for every α in L which is algebraic over K, the minimal polynomial of α over K is a separable polynomial. Inseparable extensions (that is, extensions which are not separable) may occur only in positive characteristic.
The extended specification lists a "Core List (required)", "Second List (often used)" and "Third List (suggested extensions)" of property names. [2] The word list indicates that the data is a list of values, the first of which is the number of entries in the list (represented as a 'uchar' in this case). In this example each list entry is ...
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