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Microfluidic structures include micropneumatic systems, i.e. microsystems for the handling of off-chip fluids (liquid pumps, gas valves, etc.), and microfluidic structures for the on-chip handling of nanoliter (nl) and picoliter (pl) volumes. [68] To date, the most successful commercial application of microfluidics is the inkjet printhead. [69]
Lab disk for protein structure analysis via small-angle X-ray scattering. The centrifugal micro-fluidic biochip or centrifugal micro-fluidic biodisk is a type of lab-on-a-chip technology, also known as lab-on-a-disc, that can be used to integrate processes such as separating, mixing, reaction and detecting molecules of nano-size in a single piece of platform, including a compact disk or DVD.
The advantages of PCR chips include shorter thermal-cycling time, more uniform temperature which enhances yield, and portability for point-of-care applications. [39] Two challenges in microfluidic PCR chips are PCR inhibition and contamination due to the large surface-to-volume ratio increasing surface-reagent interactions. [39]
They have developed a microfluidic human-on-a-chip, culturing four different cell types to mimic four human organs: liver, lung, kidney and fat. [104] They focused on developing a standard serum-free culture media that would be valuable to all cell types included in the device. Optimized standard media are generally targeted to one specific ...
Digital microfluidics (DMF) is a platform for lab-on-a-chip systems that is based upon the manipulation of microdroplets. Droplets are dispensed, moved, stored, mixed, reacted, or analyzed on a platform with a set of insulated electrodes.
Microfluidic devices make possible the study of a single cell to a few hundred cells in a 3D environment. Comparatively, macroscopic 2D cultures have 10 4 to 10 7 cells on a flat surface. [10] Microfluidics also allow for chemical gradients, the continuous flow of fresh media, high through put testing, and direct output to analytical ...
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