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  2. Dideoxynucleotide - Wikipedia

    en.wikipedia.org/wiki/Dideoxynucleotide

    That is, each nucleotide base of that particular type has a probability of being bonded to not a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation. Therefore, if the sample then undergoes electrophoresis, there will be a band present for each length at which the complement of the dideoxynucleotide is present.

  3. Nucleic acid sequence - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_sequence

    Given the two 10-nucleotide sequences, line them up and compare the differences between them. Calculate the percent difference by taking the number of differences between the DNA bases divided by the total number of nucleotides. In this case there are three differences in the 10 nucleotide sequence. Thus there is a 30% difference.

  4. Sanger sequencing - Wikipedia

    en.wikipedia.org/wiki/Sanger_sequencing

    Other useful applications of DNA sequencing include single nucleotide polymorphism (SNP) detection, single-strand conformation polymorphism (SSCP) heteroduplex analysis, and short tandem repeat (STR) analysis. Resolving DNA fragments according to differences in size and/or conformation is the most critical step in studying these features of the ...

  5. Nucleoside triphosphate - Wikipedia

    en.wikipedia.org/wiki/Nucleoside_triphosphate

    A nucleoside triphosphate is a nucleoside containing a nitrogenous base bound to a 5-carbon sugar (either ribose or deoxyribose), with three phosphate groups bound to the sugar. [1]

  6. Sequence analysis - Wikipedia

    en.wikipedia.org/wiki/Sequence_analysis

    Thus, sequence analysis can be used to assign function to coding and non-coding regions in a biological sequence usually by comparing sequences and studying similarities and differences. Nowadays, there are many tools and techniques that provide the sequence comparisons (sequence alignment) and analyze the alignment product to understand its ...

  7. SNP genotyping - Wikipedia

    en.wikipedia.org/wiki/SNP_genotyping

    SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. It is a form of genotyping, which is the measurement of more general genetic variation. SNPs are one of the most common types of genetic variation.

  8. Molecular phylogenetics - Wikipedia

    en.wikipedia.org/wiki/Molecular_phylogenetics

    Molecular phylogenetics (/ m ə ˈ l ɛ k j ʊ l ər ˌ f aɪ l oʊ dʒ ə ˈ n ɛ t ɪ k s, m ɒ-, m oʊ-/ [1] [2]) is the branch of phylogeny that analyzes genetic, hereditary molecular differences, predominantly in DNA sequences, to gain information on an organism's evolutionary relationships. From these analyses, it is possible to determine ...

  9. Terminal deoxynucleotidyl transferase - Wikipedia

    en.wikipedia.org/wiki/Terminal_deoxynucleotidyl...

    TdT has also seen recent application in the De Novo synthesis of oligonucleotides, with TdT-dNTP tethered analogs capable of primer extension by 1 nt at a time. [31] In other words, the enzyme TdT has demonstrated the capability of making synthetic DNA by adding one letter at a time to a primer sequence.