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Typical dot blot membrane. Darker dots indicate more protein. A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Instead, the sample is applied directly on ...
A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available. [1]
Protein:protein interaction studies; Colony blot, dot blot, Western blot and ELISA; Screening for positive expression clones; Immunocytochemistry and Immunohistochemistry; Protein localization and targeting studies; Because the Strep-tag is capable of isolating protein complexes, strategies for the study of protein-protein interactions can also ...
Former protocols were hampered by the need for large amounts of proteins and their susceptibility to degradation while being isolated. Southwestern blotting was first described by Brian Bowen, Jay Steinberg, U.K. Laemmli, and Harold Weintraub in 1979. [2] During the time the technique was originally called "protein blotting".
A northern blot is a similar analytical technique that, instead of detecting a protein of interest, is used to study gene expression by detection of RNA (or isolated mRNA) on a similar membrane. The northwestern blot combines the two techniques, and specifically involves the identification of labeled RNA that interact with proteins that are ...
In a dot blot, macromolecules are applied directly to the matrix. Macromolecules can also be separated and transferred via gel electrophoresis. [1] One of the most common blotting matrices for protein analysis is nitrocellulose, which has a high affinity for proteins due to hydrophobic interactions. However, proteins with low molecular weight ...