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Intracytoplasmic sperm injection (ICSI / ˈ ɪ k s i / IK-see) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg. This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus.
The main stages at which embryo transfer is performed are cleavage stage (day 2 to 4 after co-incubation) or the blastocyst stage (day 5 or 6 after co-incubation). [ 1 ] Embryos which reach the day 3 cell stage can be tested for chromosomal or specific genetic defects prior to possible transfer by preimplantation genetic diagnosis (PGD).
In vitro fertilisation (IVF) is a process of fertilisation in which an egg is combined with sperm in vitro ("in glass"). The process involves monitoring and stimulating a woman's ovulatory process, then removing an ovum or ova (egg or eggs) from her ovaries and enabling a man's sperm to fertilise them in a culture medium in a laboratory.
Assisted reproductive technology (ART) includes medical procedures used primarily to address infertility.This subject involves procedures such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and cryopreservation of gametes and embryos, and the use of fertility medication.
Embryo transfer refers to a step in the process of assisted reproduction in which embryos are placed into the uterus of a female with the intent to establish a pregnancy.This technique - which is often used in connection with in vitro fertilization (IVF) - may be used in humans or in other animals, in which situations and goals may vary.
Natural Cycle IVF is in vitro fertilisation (IVF) using either of the following procedures: IVF without the use any ovarian hyperstimulation drugs. [1] IVF using an ovarian hyperstimulation protocol with a GnRH antagonist for ovulation suppression, generally with gonadotropins as well. [2] This procedure can be called modified natural cycle-IVF ...
The history of in vitro fertilisation (IVF) goes back more than half a century. In 1959 the first birth in a nonhuman mammal resulting from IVF occurred, and in 1978 the world's first baby conceived by IVF was born. As medicine advanced, IVF was transformed from natural research to a stimulated clinical treatment.
In current state of the art, early embryos having undergone cryopreservation implant at the same rate as equivalent fresh counterparts. [2] The outcome from using cryopreserved embryos has uniformly been positive with no increase in birth defects or development abnormalities, [3] [8] also between fresh versus frozen eggs used for intracytoplasmic sperm injection (ICSI). [9]