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Paper chromatography is an analytical method used to separate coloured chemicals or substances. [1] It is now primarily used as a teaching tool, having been replaced in the laboratory by other chromatography methods such as thin-layer chromatography (TLC). The setup has three components. The mobile phase is a solution that travels up the ...
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
Erwin Chargaff (11 August 1905 – 20 June 2002) was an Austro-Hungarian-born American biochemist, writer, and professor of biochemistry at Columbia University medical school. [1] A Bucovinian Jew who emigrated to the United States during the Nazi regime, he penned a well-reviewed [2][3] autobiography, Heraclitean Fire: Sketches from a Life ...
Retardation factor. In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system. [1] In planar chromatography in particular, the retardation factor RF is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front. [2]
Thin-layer chromatography. Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2] The sample is deposited on the plate, which ...
Xanthophyll. The characteristic color of egg yolk results from the presence of a xanthophyll pigment typical in color of lutein or zeaxanthin of the xanthophylls, a division of the carotenoids group. Xanthophylls (originally phylloxanthins) are yellow pigments that occur widely in nature and form one of two major divisions of the carotenoid ...
The Nirenberg and Leder experiment was a scientific experiment performed in 1964 by Marshall W. Nirenberg and Philip Leder. The experiment elucidated the triplet nature of the genetic code and allowed the remaining ambiguous codons in the genetic code to be deciphered. In this experiment, using a ribosome binding assay called the triplet ...
Chromatography, literally "color writing", [1] was used—and named— in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll (which is green) and carotenoids (which are orange and yellow). New forms of chromatography developed in the 1930s and 1940s made the technique useful for a wide range ...