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In "one-step" methods, all the dyes—with or without a polyacid—are combined in a single solution. One of the oldest single-step approaches to trichrome staining is van Gieson's method, which stains muscle and cytoplasm yellow, and collagen red. Another is the Gömöri trichrome stain, which closely mimics Masson's trichrome. In "yellowsolve ...
Masson's trichrome is a three-colour staining procedure used in histology. The recipes emerged from Claude L. Pierre Masson 's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue .
Lillie's trichrome is a combination of dyes used in histology. It is similar to Masson's trichrome stain , but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [ 1 ]
Trichrome stains are staining methods in which three anionic dyes are used, in conjunction with either phosphomolybdic acid (PMA), phosphotungstic acid (PTA), or a mixture of these heteropolyacids. Probably the first trichrome method was that of Frank B Mallory, an American pathologist, first published in 1900. [1]
Mallory's trichrome stain also called Mallory's Triple Stain is a stain utilized in histology to aid in revealing different macromolecules that make up the cell. It uses the three stains: aniline blue , acid fuchsin , and orange G .
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Acid fuchsin has wide use in histology, [1] and is one of the dyes used in Masson's trichrome stain. [2] This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue ...
Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome, [5] as well as to reveal callose structures in plant tissues. [6] It can also be used in other connective tissue stains, such as Mallory's stain, [5] Gömöri trichrome stain, and Carstair's Method. [7] It is used in differential staining.