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The cellular processes of DNA replication and transcription involve DNA and RNA synthesis, respectively. DNA synthesis uses dNTPs as substrates, while RNA synthesis uses rNTPs as substrates. [2] NTPs cannot be converted directly to dNTPs. DNA contains four different nitrogenous bases: adenine, guanine, cytosine and thymine.
A ribonucleotide tri-phosphate (rNTP) is composed of a ribose sugar, 3 phosphate groups attached via diester bonds to the 5' oxygen on the ribose and a nitrogenous base attached to the 1' carbon on the ribose. rNTP's are also referred to as NTPs while the deoxyribose version is referred to as dNTPs.
In enzymology, a nucleoside-triphosphatase (NTPase) (EC 3.6.1.15) is an enzyme that catalyzes the chemical reaction. NTP + H 2 O NDP + phosphate. Thus, the two substrates of this enzyme are NTP and H 2 O, whereas its two products are NDP and phosphate.
Whisper is a machine learning model for speech recognition and transcription, created by OpenAI and first released as open-source software in September 2022. [2]It is capable of transcribing speech in English and several other languages, and is also capable of translating several non-English languages into English. [1]
An NTP binding site is a type of binding site found in nucleoside monophosphate (NMP) kinases, N can be adenosine or guanosine.A P-loop is one of the structural motifs common for nucleoside triphosphate (NTP) binding sites, it interacts with the bound nucleotide's phosphoryl groups.
Audio or video files can be transcribed manually or automatically. [1] Transcriptionists can replay a recording several times in a transcription editor and type what they hear. By using transcription hot keys, the manual transcription can be accelerated, the sound filtered, equalized or have the tempo adjusted when the clarity is not great.
Dideoxynucleotides are useful in the sequencing of DNA in combination with electrophoresis.A DNA sample that undergoes PCR (polymerase chain reaction) in a mixture containing all four deoxynucleotides and one dideoxynucleotide will produce strands of length equal to the position of each base of the type that complements the type having a dideoxynucleotide present.
Transcription preinitiation complex, represented by the central cluster of proteins, causes RNA polymerase to bind to target DNA site. The PIC is able to bind both the promoter sequence near the gene to be transcribed and an enhancer sequence in a different part of the genome, allowing enhancer sequences to regulate a gene distant from it.