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Dishcloths are often left damp and provide a breeding ground for bacteria. Since the kitchen sink is used to clean food, dishcloths are routinely infected with E. coli and salmonella. In 2007, a study from the Journal of Environmental Health found that putting a damp dishcloth (or sponge) in the microwave for 2 minutes killed 99% of living ...
The result is a stem-loop primer that excludes annealing involving shorter overlaps, but permits annealing of the primer to its fully complementary sequence in the target. Chimeric primers: some DNA bases in the primer are replaced with RNA bases, creating a chimeric sequence. The melting temperature of a chimeric sequence with another chimeric ...