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With bafilomycin, faster contraction and relaxation of the aorta was seen as bafilomycin prevented the ion trapping of xylometazoline in the lysosome. Without pre-treatment with bafilomycin, the functional V-ATPase causes the lysosome to become a reservoir for xylometazoline, slowing its effect on contractility.
The yeast V-ATPase is the best characterized. There are at least thirteen subunits identified to form a functional V-ATPase complex, which consists of two domains. The subunits belong to either the V o domain (membrane associated subunits, lowercase letters on the figure) or the V 1 domain (peripherally associated subunits, uppercase letters on the figure).
[citation needed] Acridine orange is also used to stain acidic vacuoles (lysosomes, endosomes, and autophagosomes), RNA, and DNA in living cells. This method is a cheap and easy way to study lysosomal vacuolation, autophagy, and apoptosis. The emission color of acridine orange changes from yellow, to orange, to red as the pH drops in an acidic ...
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A test for lipase using polysorbate 80 (polyoxyethylene sorbitan monooleate, a detergent). Certain mycobacteria possess a lipase that splits it into oleic acid and polyoxyethylated sorbitol . The test solution also contains phenol red , which is stabilised by the polysorbate 80; when the latter 80 is hydrolysed, the phenol red changes from ...
A test method is a method for a test in science or engineering, such as a physical test, chemical test, or statistical test. It is a definitive procedure that produces a test result. [ 1 ] In order to ensure accurate and relevant test results, a test method should be "explicit, unambiguous, and experimentally feasible.", [ 2 ] as well as ...
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