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Layering is a vegetative propagation technique where the stem or branch of a plant is manipulated to promote root development while still attached to the parent plant. Once roots are established, the new plant can be detached from the parent and planted. Layering is utilized by horticulturists to propagate desirable plants.
In plant tissue culture IBA and other auxins are used to initiate root formation in vitro in a procedure called micropropagation.Micropropagation of plants is the process of using small samples of plants called explants and causing them to undergo growth of differentiated or undifferentiated cells.
NAA is a synthetic plant hormone in the auxin family and is an ingredient in many commercial horticultural products; it is a rooting agent and used for the vegetative propagation of plants from stem and leaf cuttings. It is also used for plant tissue culture. [2]
In horticulture, auxins, especially NAA and IBA, are commonly applied to stimulate root initiation when rooting cuttings of plants. However, high concentrations of auxin inhibit root elongation and instead enhance adventitious root formation. Removal of the root tip can lead to inhibition of secondary root formation.
Root mucilage is a part of a wider secrete from plant roots known as root exudate. Plant roots secrete a variety of organic molecules into the surrounding soil, such as proteins, enzymes, DNA, sugars and amino acids, which are the building blocks of life. [3] [4] This collective secretion is known as root exudate.
The propagation of plants by cuttings of fully developed leaves, stems, or roots is performed by gardeners utilizing auxin as a rooting compound applied to the cut surface; the auxins are taken into the plant and promote root initiation.