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The T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression. [3] [2] T7 has been used as a model in synthetic biology. Chan et al. (2005) "refactored" the genome of T7, replacing approximately 12 kbp of its genome with engineered DNA. [15]
In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different polymerases.
Italiano: Rappresentazione schematica della struttura del fago T7. Español: Representación esquemática de la estructura del fago T7. Français : Représentation schématique de la structure de la phage T7.
Once this has occurred, the prohead undergoes maturation by cleavage of capsid subunits to form an icosahedral phage head with 5-fold symmetry. After the head maturation, the tail is joined in one of two ways: Either the tail is constructed separately, and joined with the connector, or the tail is constructed directly onto the phage head.
T7 phage group was renamed to T7-like phages in the sixth report in 1995. In 1998, the whole family was moved into the newly created order Caudovirales , and the genus was renamed again in the seventh report in 1999 to T7-like viruses . 2009 saw the genus moved into the newly created subfamily Autographivirinae , and it was renamed again in ...
The T7 expression system is used in the field of microbiology to clone recombinant DNA using strains of E. coli. [1] It is the most popular system for expressing recombinant proteins in E. coli. [2] By 2021, this system had been described in over 220,000 research publications. [3]
Podoviridae was a family of bacteriophage in the order Caudovirales often associated with T-7 like phages. [1] The family and order Caudovirales have now been abolished, with the term podovirus now used to refer to the morphology of viruses in this former family. [2]
While phage T7 mediates DNA replication in very similar manner to higher organisms, T7 system is generally simpler compared to other replication systems. In addition to T7 DNA polymerase (also known as gp5), T7 replisome requires only four accessory proteins for proper function: host thioredoxin, gp4, gp2.5, and gp1.7.