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A 2010 episode of Law & Order, "Immortal", was heavily based on the story of Henrietta Lacks and the HeLa cell line, using the fictional "NaRo" cells as a stand-in. [68] The story of how the HeLa cell line came to be was also the subject of a 2010 episode of the podcast Radiolab. [69]
An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods in vitro. The mutations required for immortality can occur ...
One significant cell-line cross contaminant is the immortal HeLa cell line. HeLa contamination was first noted in the early 1960s in non-human culture in the USA. Intraspecies contamination was discovered in nineteen cell lines in the seventies. In 1974, five human cell lines from the Soviet Union were found to be HeLa.
The cells will often have a known range of confluencies for optimal growth, for example a mammalian cell line like HeLa generally prefers confluencies between 10% and 100%, and subculture will normally try to keep the cells in this range.
Baculovirus-infected insect cells [20] (Sf9, Sf21, High Five strains) or mammalian cells [21] (HeLa, HEK 293) allow production of glycosylated or membrane proteins that cannot be produced using fungal or bacterial systems. [20] [6] It is useful for production of proteins in high quantity. Genes are not expressed continuously because infected ...
However, this results in a false positive for cells that naturally have these two proteins such as maturing tissue macrophages with senescence-associated beta-galactosidase and T-cells with p16 Ink4A. [13] Senescent cells can undergo conversion to an immunogenic phenotype that enables them to be eliminated by the immune system. [18]
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