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The T7 gene is itself under the control of a lac promoter. Normally, both the lac promoter and the T7 promoter are repressed in the E. coli cell by the Lac repressor. In order to initiate transcription, an inducer must bind to the lac repressor and prevent it from inhibiting the gene expression of the T7 gene. Once this happens, the gene can be ...
These explain how T7 polymerase binds to DNA and transcribes it. The N-terminal domain moves around as the elongation complex forms. The ssRNAP holds a DNA-RNA hybrid of 8bp. [3] A beta-hairpin specificity loop (residues 739-770 in T7) recognizes the promoter; swapping it out for one found in T3 RNAP makes the polymerase recognize T3 promoters ...
Of the USCIS immigration forms, decisions on the two forms Form I-130 (family-based immigration, the F and IR categories) and the widower subcategory for Form I-360 (special immigrants, the EB-4 category), must be appealed through the EOIR-29 (Notice of Appeal to the Board of Immigration Appeals from a Decision of an Immigration Officer) to the ...
T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase “reads” existing DNA strands and creates two new strands that match the existing ones. The T7 DNA polymerase requires a host factor, E. coli thioredoxin, [1] in order to carry out its function
In a 1945 study by Demerec and Fano, [4] T7 was used to describe one of the seven phage types (T1 to T7) that grow lytically on Escherichia coli. [5] Although all seven phages were numbered arbitrarily, phages with odd numbers, or T-odd phages, were later discovered to share morphological and biochemical features that distinguish them from T-even phages. [6]
The thoracic spinal nerve 7 (T7) is a spinal nerve of the thoracic segment. [ 1 ] It originates from the spinal column from below the thoracic vertebra 7 (T7).
A ternary flammability diagram, showing which mixtures of methane, oxygen gas, and inert nitrogen gas will burn. A ternary plot, ternary graph, triangle plot, simplex plot, or Gibbs triangle is a barycentric plot on three variables which sum to a constant. [1]
Thermal conductivities have been measured with longitudinal heat flow methods where the experimental arrangement is so designed to accommodate heat flow in only the axial direction, temperatures are constant, and radial heat loss is prevented or minimized.