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2. Spin column-based nucleic acid purification - Wikipedia

   en.wikipedia.org/wiki/Spin_column-based_nucleic...

   The different stages of the method are lyse, bind, wash, and elute. [1] [2] More specifically, this entails the lysis of target cells to release nucleic acids, selective binding of nucleic acid to a silica membrane, washing away particulates and inhibitors that are not bound to the silica membrane, and elution of the nucleic acid, with the end result being purified nucleic acid in an aqueous ...

3. DNA extraction - Wikipedia

   en.wikipedia.org/wiki/DNA_extraction

   DNA storage is an important aspect of DNA extraction projects as it ensures the integrity and stability of the extracted DNA for downstream applications. [ 15 ] One common method of DNA storage is ethanol precipitation, which involves adding ethanol and a salt, such as sodium chloride or potassium acetate, to the extracted DNA to precipitate it ...

4. Ethanol precipitation - Wikipedia

   en.wikipedia.org/wiki/Ethanol_precipitation

   Isopropanol can also be used instead of ethanol; the precipitation efficiency of the isopropanol is higher making one volume enough for precipitation. However, isopropanol is less volatile than ethanol and needs more time to air-dry in the final step. The pellet might also adhere less tightly to the tube when using isopropanol. [2]

5. Nucleic acid methods - Wikipedia

   en.wikipedia.org/wiki/Nucleic_acid_methods

   DNA extraction; Phenol–chloroform extraction ... Protocols for Recombinant DNA Isolation, Cloning, and Sequencing This page was last edited on ... By using this ...

6. Gel extraction - Wikipedia

   en.wikipedia.org/wiki/Gel_extraction

   Gel extraction kits are available from several major biotech manufacturers for a final cost of approximately 1–2 US$ per sample. Protocols included in these kits generally call for the dissolution of the gel-slice in 3 volumes of chaotropic agent at 50 °C, followed by application of the solution to a spin-column (the DNA remains in the column), a 70% ethanol wash (the DNA remains in the ...

7. DNA separation by silica adsorption - Wikipedia

   en.wikipedia.org/wiki/DNA_separation_by_silica...

   In order to separate DNA through silica adsorption, a sample is first lysed, releasing proteins, DNA, phospholipids, etc. from the cells. The remaining tissue is discarded. The supernatant containing the DNA is then exposed to silica in a solution with high ionic strength. The highest DNA adsorption efficiencies occur in the presence of buffer ...