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The comprehensive metabolic panel, or chemical screen (CMP; CPT code 80053), is a panel of 14 blood tests that serves as an initial broad medical screening tool. The CMP provides a rough check of kidney function, liver function, diabetic and parathyroid status, and electrolyte and fluid balance, but this type of screening has its limitations.
An anti-α-amylase antibody is fixed at the test line. If human salivary amylase is present in the sample, it will form an antigen-antibody-colloidal gold complex upon deposition. These complexes react with the antibodies fixed at the test line to form a visible red line indicating the presence of human salivary α-amylase, and therefore, human ...
A presumptive test to detect saliva is the alpha-amylase test also known as the Phadebas Test. [4] This detection technique is based on the activity of the enzyme alpha-amylase which breaks down starches from food into smaller oligosaccharide molecules, starting digestion in the mouth. [ 11 ]
Macroamylasemia is a relatively rare condition, and in most cases, the exact cause is unknown. It can occur in completely healthy individuals without symptoms, in association with autoimmune diseases (such as systemic lupus erythematosus) and other diseases (cancer or inflammatory diseases).
Prepare a series of standards diluted with 0.15 M NaCl to final concentrations of 0 (blank = No protein), 250, 500, 750 and 1500 μg/mL. Also prepare serial dilutions of the unknown sample to be measured. Add 100 μL of each of the above to a separate test tube (or spectrophotometer tube if using a Spectronic 20).
Alpha-amylase 1 is an enzyme that in humans is encoded by the AMY1A gene. [3] This gene is found in many organisms. Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and g
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The starch iodine test, a development of the iodine test, is based on colour change, as α-amylase degrades starch and is commonly used in many applications. A similar but industrially produced test is the Phadebas amylase test, which is used as a qualitative and quantitative test within many industries, such as detergents, various flour, grain ...