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Fig. 1: Underwater plants in a fish tank, and their inverted images (top) formed by total internal reflection in the water–air surface. In physics, total internal reflection (TIR) is the phenomenon in which waves arriving at the interface (boundary) from one medium to another (e.g., from water to air) are not refracted into the second ("external") medium, but completely reflected back into ...
A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.
It is known that the value of lateral position Goos–Hänchen shift is only 5–10 μm at a total internal reflection interface of water and air, which is very difficult to be experimentally measured.
Total internal reflection microscopy is a specialized optical imaging technique for object tracking and detection utilizing the light scattered from an evanescent field in the vicinity of a dielectric interface. Its advantages are a high signal-to-noise ratio and a high spatial resolution in the vertical dimension.
Fig. 2: Phase advance at "internal" reflections for refractive indices of 1.55, 1.5, and 1.45 ("internal" relative to "external"). Beyond the critical angle, the p (red) and s (blue) polarizations undergo unequal phase shifts on total internal reflection; the macroscopically observable difference between these shifts is plotted in black.
The area outside Snell's window will either be completely dark or show a reflection of underwater objects by total internal reflection. Underwater photographers sometimes compose photographs from below such that their subjects fall inside Snell's window, which backlights and focuses attention on the subjects.
The phase shift of the reflected wave on total internal reflection can similarly be obtained from the phase angles of r p and r s (whose magnitudes are unity in this case). These phase shifts are different for s and p waves, which is the well-known principle by which total internal reflection is used to effect polarization transformations.
Optical phenomena encompass a broad range of events, including those caused by atmospheric optical properties, other natural occurrences, man-made effects, and interactions involving human vision (entoptic phenomena).