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There are four basic steps of the Gram stain: Applying a primary stain ( crystal violet ) to a heat-fixed smear of a bacterial culture. Heat fixation kills some bacteria but is mostly used to affix the bacteria to the slide so that they do not rinse out during the staining procedure.
Microbial cultures are foundational and basic diagnostic methods used as research tools in molecular biology. The term culture can also refer to the microorganisms being grown. Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both.
Microtechnique is an aggregate of methods used to prepare micro-objects for studying. [1] It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique.
Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...
The dip slide test consists of a sterile culture medium on a plastic carrier that is dipped into the liquid to be sampled. [3] The culture is then incubated, allowing for microbial growth. [2] Most Dip slides consist of 1 - 2 agars attached to a flexible plastic paddle, this allows full contact of the agar onto the desired area for testing. [4]
Serological test slides like those for ASO, VDRL, rheumatoid factor: provide links: Specimen Dish: used to hold specimen or samples Sterile loops: used to inoculate test samples into culture media for bacterial or fungal cultures, antibiograms, etc.; not heated before use—these are disposable pre-sterilised Thermal cycler
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