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Molisch test (using α-napthol) indicating a positive result (see purple ring). Molisch's test is a sensitive chemical test, named after Austrian botanist Hans Molisch, for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol (usually α-naphthol, though other ...
Hyperglycemic clamp technique: The plasma glucose concentration is acutely raised to 125 mg/dl above basal levels by a continuous infusion of glucose. This hyperglycemic plateau is maintained by adjustment of a variable glucose infusion, based on the rate of insulin secretion and glucose metabolism. Because the plasma glucose concentration is ...
But following meals, capillary and arterial blood glucose levels can be significantly higher than venous levels. Although these differences vary widely, one study found that following the consumption of 50 grams of glucose, "the mean capillary blood glucose concentration is higher than the mean venous blood glucose concentration by 35%." [33] [34]
Glucose concentrations vary in an individual, and a healthy person can present with transitory glucosuria after a meal high in sugars; therefore the most representative results come from samples obtained at least two hours after food is eaten. The detection of glucose by test strips is based on the enzymatic reaction of glucose oxidase.
Besides glucose oxidase, the test kit contains a benzidine derivative, which is oxidized to a blue polymer by the hydrogen peroxide formed in the oxidation reaction. The disadvantage of this method was that the test strip had to be developed after a precise interval (the blood had to be washed away), and the meter needed to be calibrated ...
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Blood glucose monitoring is the use of a glucose meter for testing the concentration of glucose in the blood . Particularly important in diabetes management , a blood glucose test is typically performed by piercing the skin (typically, via fingerstick ) to draw blood, then applying the blood to a chemically active disposable 'test-strip'.
Substance in water + 3 mL Benedict's solution, then boil for few minutes and allow to cool. Red, green, or yellow precipitate is obtained Reducing sugar, such as glucose, is present Substance in water + 3 mL Benedict's solution, then boil for few minutes and allow to cool. Solution remains clear or is a little blue Reducing sugar is not present