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Unfortunately, none of Mallory's publications (they go from 1891 [2] to 1938 [3]) provide any explanation of the rationales of either his trichrome or his phosphotungstic acid-haematoxylin (PTAH) method. Nobody knows why Mallory introduced heteropolyacids into microtechnique.
Principally used as a nuclear stain (to stain the cell nucleus), haematoxylin will also stain rough endoplasmic reticulum, ribosomes, collagen, myelin, elastic fibers, and acid mucins. [10] Haematoxylin alone is not an effective stain, but when oxidized to hematein, and combined with a mordant, stains chromatin in cell nuclei dark blue to black.
Another is the Gömöri trichrome stain, which closely mimics Masson's trichrome. In "yellowsolve" methods, a red dye in dilute acetic acid is first applied, then the section is very thoroughly dehydrated to ensure that no moisture remains. The red dye is then displaced by a yellow dye in a solvent, such as cellosolve (2-ethoxy-ethanol).
Another common variant is the Masson trichrome & Verhoeff stain, which combines the Masson trichrome stain and Verhoeff's stain. [2] This combination is useful for the examination of blood vessels ; the Verhoeff stain highlights elastin (black) and allows one to easily differentiate small arteries (which typically have at least two elastic ...
Poly(p-phenylene oxide) (PPO), poly(p-phenylene ether) (PPE), poly(oxy-2,6-dimethyl-1,4-phenylene), often referred to simply as polyphenylene oxide, is a high-temperature thermoplastic with the general formula (C 8 H 8 O) n. It is rarely used in its pure form due to difficulties in processing.
Colours on sculptures, coffins, and architecture had both aesthetic and symbolic qualities. Ancient Egyptians saw black as the colour of the fertile alluvial soil, and so associated it with fertility and regeneration. Black was also associated with the afterlife, and was the colour of funerary deities like Anubis. White was the colour of purity ...