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For cameras that can only focus on one object distance at a time, depth of field is the distance between the nearest and the farthest objects that are in acceptably sharp focus in the image. [1] ". Acceptably sharp focus" is defined using a property called the "circle of confusion". The depth of field can be determined by focal length, distance ...
Aperture. In biology, the pupil (appearing as a black hole) of the eye is its aperture and the iris is its diaphragm. In humans, the pupil can constrict to as small as 2 mm (f/ 8.3) and dilate to larger than 8 mm (f/ 2.1) in some individuals. In optics, the aperture of an optical system (including a system consisted of a single lens) is a hole ...
The depth of field of an image produced at a given f-number is dependent on other parameters as well, including the focal length, the subject distance, and the format of the film or sensor used to capture the image. Depth of field can be described as depending on just angle of view, subject distance, and entrance pupil diameter (as in von Rohr ...
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives
Depth of field when the PoF is rotated When the lens and image planes are parallel, the depth of field (DoF) extends between parallel planes on either side of the plane of focus. When the Scheimpflug principle is employed, the DoF becomes wedge shaped (Merklinger 1996, 32; Tillmanns 1997, 71), [ f ] with the apex of the wedge at the PoF ...
Scale bars: 1 μm. [ 2 ] Near-field scanning optical microscopy (NSOM) or scanning near-field optical microscopy (SNOM) is a microscopy technique for nanostructure investigation that breaks the far field resolution limit by exploiting the properties of evanescent waves. In SNOM, the excitation laser light is focused through an aperture with a ...
Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).
Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy. Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.