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The enzyme is used in biotechnology as a meganuclease. It recognises an 18-base pair sequence TAGGGATAACAGGGTAAT and leaves a 4 base pair 3' hydroxyl overhang. It is a rare cutting endonuclease. Statistically an 18-bp sequence will occur once in every 6.9*10 10 base pairs (a frequency of 1 in 4 18). This sequence does not normally occur in a ...
REBASE contains an extensive set of references, sites of recognition and cleavage, sequences and structures. It also contains information on the commercial availability of each enzyme. REBASE is one of the longest running biological databases having its roots in a collection of restriction enzymes maintained by Richard J. Roberts since before ...
The restriction modification system (RM system) is found in bacteria and archaea, and provides a defense against foreign DNA, such as that borne by bacteriophages.. Bacteria have restriction enzymes, also called restriction endonucleases, which cleave double-stranded DNA at specific points into fragments, which are then degraded further by other endonucleases.
A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. [1] [2] [3] Restriction enzymes are one class of the broader endonuclease group of enzymes.
It is a restriction enzyme used in molecular biology laboratories. It was the third endonuclease to be isolated from the Haemophilus aegyptius bacteria . The enzyme's recognition site—the place where it cuts DNA molecules—is the GGCC nucleotide sequence which means it cleaves DNA at the site 5′-GG/CC-3.
This article contains a list of restriction enzymes whose names start with A and have a clearly defined cutting site. The following information is given for each enzyme: Name of Restriction Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature, [1] [2] and bibliographical references. Note: When ...
In molecular biology, the restriction endonuclease BsobI/AvaI family of enzymes includes the AvaI and BsoBI restriction endonucleases from Anabaena variabilis and Bacillus stearothermophilus, both of which recognise the double-stranded sequence CYCGRG (where Y = T/C, and R = A/G) and cleave after C-1.
This article contains a list of the most studied restriction enzymes whose names start with L to N inclusive. It contains approximately 120 enzymes. The following information is given: Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature [1] [2], and bibliographical references.