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Depth of field depends on the focus distance, while depth of focus does not. Depth of focus can have two slightly different meanings. The first is the distance over which the image plane can be displaced while a single object plane remains in acceptably sharp focus; [1][2] [clarify] the second is the image-side conjugate of depth of field.
The hyperfocal distance has a property called "consecutive depths of field", where a lens focused at an object whose distance from the lens is at the hyperfocal distance H will hold a depth of field from H/2 to infinity, if the lens is focused to H/2, the depth of field will be from H/3 to H; if the lens is then focused to H/3, the depth of ...
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives
Comparison of f /32 (top-left half) and f /5 (bottom-right half) Shallow focus with a wide open lens. Depth of field increases with f-number, as illustrated in the image here. This means that photographs taken with a low f-number (large aperture) will tend to have subjects at one distance in focus, with the rest of the image (nearer and farther ...
The hyperfocal distance has a property called "consecutive depths of field", where a lens focused at an object whose distance from the lens is at the hyperfocal distance H will hold a depth of field from H/2 to infinity, if the lens is focused to H/2, the depth of field will be from H/3 to H; if the lens is then focused to H/3, the depth of ...
Depth of field formulas derived from geometrical optics imply that any arbitrary DoF can be achieved by using a sufficiently small CoC. Because of diffraction , however, this is not quite true. Using a smaller CoC requires increasing the lens f-number to achieve the same DoF, and if the lens is stopped down sufficiently far, the reduction in ...
Great working distance and depth of field are important qualities for this type of microscope. Both qualities are inversely correlated with resolution: the higher the resolution (i.e. the greater the distance at which two adjacent points can be distinguished as separate), the smaller the depth of field and working distance. Some stereo ...
Deep focus is a photographic and cinematographic technique using a large depth of field. Depth of field is the front-to-back range of focus in an image, or how much of it appears sharp and clear. In deep focus, the foreground, middle ground, and background are all in focus. Deep focus is normally achieved by choosing a small aperture.