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The hectograph, gelatin duplicator or jellygraph is a printing process that involves transfer of an original, prepared with special inks, to a pan of gelatin or a gelatin pad pulled tight on a metal frame. [1] While the original use of the technology has diminished, it has recently been revived for use in the art world.
Agarose gel has large pore size and good gel strength, making it suitable as an anticonvection medium for the electrophoresis of DNA and large protein molecules. The pore size of a 1% gel has been estimated from 100 nm to 200–500 nm, [ 4 ] [ 5 ] and its gel strength allows gels as dilute as 0.15% to form a slab for gel electrophoresis. [ 6 ]
Gel electrophoresis uses a gel as an anticonvective medium or sieving medium during electrophoresis. Gels suppress the thermal convection caused by the application of the electric field and can also simply serve to maintain the finished separation so that a post electrophoresis stain can be applied. [ 3 ]
The image transfer medium was originally a stencil made from waxed mulberry paper. Later this became an immersion-coated long-fiber paper, with the coating being a plasticized nitrocellulose. This flexible waxed or coated sheet is backed by a sheet of stiff card stock, with the two sheets bound at the top.
The choice of alkaline over neutral transfer methods, however, is often empirical and may result in equivalent results. [citation needed] Blotting: A sheet of nitrocellulose (or, alternatively, nylon) membrane is placed on top of (or below, depending on the direction of the transfer) the gel. Pressure is applied constantly to the gel (either ...
Picture of an SDS-PAGE. The molecular markers (ladder) are in the left lane. Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.