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CAMP test is shown at bottom left. The CAMP test (Christie–Atkins–Munch-Petersen) is a test to identify group B β-hemolytic streptococci (Streptococcus agalactiae) [1] [2] based on their formation of a substance, CAMP factor, [3] that enlarges the area of hemolysis formed by the β-hemolysin elaborated from Staphylococcus aureus.
[6] [7] After incubation (18–24 hours, 35-37 °C), the enrichment broth is subcultured overnight in blood agar plates and GBS-like colonies (big colonies, 3-4 millimeters diameter, surrounded by narrow zone of hemolysis) [6] [7] are identified by the CAMP test or using latex agglutination with GBS antiserum or MALDI-TOF. [6] [7] [12] [14]
[8] [9] The CAMP test is also another important test for identification of GBS. The CAMP factor produced by GBS acts synergistically with the staphylococcal β-hemolysin inducing enhanced hemolysis of sheep or bovine erythrocytes. [8] GBS is also able to hydrolyze hippurate and this test can also be used to identify presumptively GBS. [8]
This phenomenon is the mechanism behind the CAMP test, [2] a test that was historically used for the identification of Streptococcus agalactiae and Listeria monocytogenes. [3] A modified version of this test called the reverse CAMP test, utilizing S. agalactiae instead of S. aureus, can also be used to identify Clostridium perfringens.
A coronavirus outbreak was reported at an overnight summer camp in Georgia that did not require campers to wear face masks. The Centers for Disease Control and Prevention released a study Friday ...
The reverse CAMP test utilizes the synergetic hemolytic abilities of the CAMP factor produced by Streptococcus agalactiae with the α-toxin produced by Clostridium perfringens. Streaking these two organisms perpendicular to each other on a blood agar plate will yield a “bow-tie” clearing of the blood agar by the hemolytic capabilities of ...
This is called the CAMP test. Streptococcus agalactiae displays this property. Clostridium perfringens can be identified presumptively with this test. Listeria monocytogenes is also positive on sheep's blood agar. Alpha-hemolytic S. viridans (right) and beta-hemolytic S. pyogenes (left) streptococci growing on blood agar
Image credits: National Geographic #5. The 'Spanish Flu' actually likely got its start in Kansas, USA. It's only called the Spanish Flu because most countries involved in WWI had a near-universal ...