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  2. Cryofixation - Wikipedia

    en.wikipedia.org/wiki/Cryofixation

    Typical specimens for cryofixation include small samples of plant or animal tissue, cell suspensions of microorganisms or cultured cells, suspensions of viruses or virus capsids and samples of purified macromolecules, especially proteins. [2] [3] Types of cryo-fixation are freezing-drying, freezing-substitution and freezing-etching.

  3. Cryopreservation - Wikipedia

    en.wikipedia.org/wiki/Cryopreservation

    Controlled-rate and slow freezing, also known as slow programmable freezing (SPF), [18] is a technique where cells are cooled to around -196 °C over the course of several hours. Slow programmable freezing was developed during the early 1970s, and eventually resulted in the first human frozen embryo birth in 1984. Since then, machines that ...

  4. Cryoprotectant - Wikipedia

    en.wikipedia.org/wiki/Cryoprotectant

    A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods. Cryoprotectants are also used to ...

  5. Cryobiology - Wikipedia

    en.wikipedia.org/wiki/Cryobiology

    At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water.

  6. Cooling bath - Wikipedia

    en.wikipedia.org/wiki/Cooling_bath

    Cooling baths are generally one of two types: (a) a cold fluid (particularly liquid nitrogen, water, or even air) — but most commonly the term refers to (b) a mixture of 3 components: (1) a cooling agent (such as dry ice or ice); (2) a liquid "carrier" (such as liquid water, ethylene glycol, acetone, etc.), which transfers heat between the ...

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  8. Frozen section procedure - Wikipedia

    en.wikipedia.org/wiki/Frozen_section_procedure

    The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...

  9. Snap freezing - Wikipedia

    en.wikipedia.org/wiki/Snap_freezing

    Snap freezing (or cook-chill or blast freezing) is the process of rapid cooling of a substance for the purpose of preservation. It is widely used in the culinary and scientific industries. It is widely used in the culinary and scientific industries.