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The numerical aperture with respect to a point P depends on the half-angle, θ1, of the maximum cone of light that can enter or exit the lens and the ambient index of refraction. As a pencil of light goes through a flat plane of glass, its half-angle changes to θ2. Due to Snell's law, the numerical aperture remains the same: NA = n1sin θ1 ...
Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).
Efforts to improve resolution by increasing the numerical aperture have led to the use of immersion lithography. As further improvements in resolution through wavelength reduction or increases in numerical aperture have become either technically challenging or economically unfeasible, much attention has been paid to reducing the k1-factor.
The line-spread function is directly proportional to the vertical integration of the point-spread image. The optical-transfer function (OTF) is defined as the Fourier transform of the point-spread function and is thus generally a two-dimensional complex function. Typically only a one-dimensional slice is shown (c), corresponding to the Fourier ...
76113 Ensembl ENSG00000167419 ENSMUSG00000009356 UniProt P22079 n/a RefSeq (mRNA) NM_001160102 NM_006151 NM_080420 RefSeq (protein) NP_001153574 NP_006142 n/a Location (UCSC) Chr 17: 58.22 – 58.27 Mb Chr 11: 87.7 – 87.72 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Lactoperoxidase is a peroxidase enzyme secreted from mammary, salivary and other mucosal glands including the ...
The interference occurs for white light when the path lengths of the measurement beam and the reference beam are nearly matched. By scanning (changing) the measurement beam path length relative to the reference beam, a correlogram is generated at each pixel. The width of the resulting correlogram is the coherence length, which depends strongly ...
A point source as imaged by a system with negative (top), zero (center), and positive (bottom) spherical aberration. Images to the left are defocused toward the inside, images on the right toward the outside. The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object.
The ability of a lens to resolve detail is usually determined by the quality of the lens, but is ultimately limited by diffraction.Light coming from a point source in the object diffracts through the lens aperture such that it forms a diffraction pattern in the image, which has a central spot and surrounding bright rings, separated by dark nulls; this pattern is known as an Airy pattern, and ...