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For instance, the RCF of 1000 x g means that the centrifugal force is 1000 times stronger than the Earth's gravitational force. RCF is dependent on the speed of rotation in rpm and the distance of the particles from the center of rotation. The most common formula used for calculating RCF is: [7]
In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time t {\displaystyle t} (in hours) required for sedimentation of a fraction with a known sedimentation coefficient s {\displaystyle s} (in svedbergs ):
To avoid having to perform a mathematical calculation every time, one can find nomograms for converting RCF to rpm for a rotor of a given radius. A ruler or other straight edge lined up with the radius on one scale, and the desired RCF on another scale, will point at the correct rpm on the third scale. [32]
An ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1 000 000 g (approx. 9 800 km/s²). [1] There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge.
A tricanter centrifuge operates on a similar principle to decanter centrifuges but instead separates three phases, consisting of a suspended solids phase and two immiscible liquids. [5] Sedimentation of the suspended solids occurs as normal where they accumulate on the wall of the bowl and are conveyed out of the centrifuge.
The gas centrifuge consists of a cylindrical rotor, a casing, an electric motor, and three lines for material to travel. The gas centrifuge is designed with a casing that completely encloses the centrifuge. [4] The cylindrical rotor is located inside the casing, which is evacuated of all air to produce a near frictionless rotation when ...
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