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An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
The first culture media was liquid media, designed by Louis Pasteur in 1860. [2] This was used in the laboratory until Robert Koch's development of solid media in 1881. [3] Koch's method of using a flat plate for his solid media was replaced by Julius Richard Petri's round box in 1887. [2]
Media with a high salt concentration will select for halophiles. Selenite broth is used to selectively isolate Salmonella species. Alkaline Peptone Water is used for the cultivation of vibrio. [5] Both these examples are clinically relevant for clinical microbiology relating to stool samples.
An agar plate being viewed in an electronic colony counter Example of a workup algorithm of possible bacterial infection in cases with no specifically requested targets (non-bacteria, mycobacteria etc.), with most common situations and agents seen in a New England community hospital setting. Different agar plates are used for different specimen ...
LB medium bottle and LB agar plate Plate medium agar LB. Lysogeny broth (LB) is a nutritionally rich medium primarily used for the growth of bacteria. Its creator, Giuseppe Bertani, intended LB to stand for lysogeny broth, [1] but LB has also come to colloquially mean Luria broth, Lennox broth, life broth or Luria–Bertani medium. [2]
Nutrient broth has the same composition, but lacks agar. [ 2 ] [ 3 ] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.
For example, MH agar may be used in the laboratory for the rapid presumptive identification of C. albicans, as an alternative method for germ tube test (Mattie. As, 2014). The medium is also free of inhibitors that could interfere with bacterial growth, making it a reliable and consistent substrate for bacterial cultures.
The third method is cell culture, of which there are three types: (1) precursor cell culture, i.e. undifferentiated cells that are to be differentiate, (2) differentiated cell culture, i.e. completely differentiated cells that have lost the capacity to further differentiate, and (3) stem cell culture, i.e. undifferentiated cells that can ...