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  2. Ultraviolet–visible spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Ultraviolet–visible...

    UV–visible spectroscopy of microscopic samples is done by integrating an optical microscope with UV–visible optics, white light sources, a monochromator, and a sensitive detector such as a charge-coupled device (CCD) or photomultiplier tube (PMT). As only a single optical path is available, these are single beam instruments.

  3. Spectrophotometry - Wikipedia

    en.wikipedia.org/wiki/Spectrophotometry

    Ultraviolet-visible (UV-vis) spectroscopy involves energy levels that excite electronic transitions. Absorption of UV-vis light excites molecules that are in ground-states to their excited-states. [5] Visible region 400–700 nm spectrophotometry is used extensively in colorimetry science. It is a known fact that it operates best at the range ...

  4. Woodward's rules - Wikipedia

    en.wikipedia.org/wiki/Woodward's_rules

    With the aid of these rules the UV absorption maximum can be predicted, for example in these two compounds: [8] In the compound on the left, the base value is 214 nm (a heteroannular diene). This diene group has 4 alkyl substituents (labeled 1,2,3,4) and the double bond in one ring is exocyclic to the other (adding 5 nm for an exocyclic double ...

  5. Optical spectrometer - Wikipedia

    en.wikipedia.org/wiki/Optical_spectrometer

    In modern spectrographs in the UV, visible, and near-IR spectral ranges, the spectrum is generally given in the form of photon number per unit wavelength (nm or μm), wavenumber (μm −1, cm −1), frequency (THz), or energy (eV), with the units indicated by the abscissa.

  6. UV-Vis absorption spectroelectrochemistry - Wikipedia

    en.wikipedia.org/wiki/UV-Vis_absorption_spectro...

    It is the link between the electrochemistry and the UV-Vis absorption spectroscopy. [3] Devices to conduct the radiation beam: lenses, mirrors and/or optical fibers. The last ones conduct electromagnetic radiation over great distances with hardly any losses.

  7. Molecular electronic transition - Wikipedia

    en.wikipedia.org/wiki/Molecular_electronic...

    The electronic transitions in organic compounds and some other compounds can be determined by ultraviolet–visible spectroscopy, provided that transitions in the ultraviolet (UV) or visible range of the electromagnetic spectrum exist for the compound.