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A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
A Gram stain of mixed Staphylococcus aureus (S. aureus ATCC 25923, gram-positive cocci, in purple) and Escherichia coli (E. coli ATCC 11775, gram-negative bacilli, in red), the most common Gram stain reference bacteria. Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups ...
Numerous naturally-occurring stains exist, such as rust on iron and a patina on bronze, [3] as do accidental stains such as from ketchup and oil on fabrics and other materials. Different types of material can be stained by different substances, and stain resistance is an important characteristic in modern textile engineering .
The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. In a more strict sense, the term vital staining has a meaning contrasting with supravital staining.
In Berlin, in 1884, Gram developed a method for distinguishing between two major classes of bacteria. [1] This technique, known as Gram staining, continues to be a standard procedure of medical microbiology. This work gained Gram an international reputation. The staining method later played a major role in classifying bacteria. Gram was a ...
In "one-step" methods, all the dyes—with or without a polyacid—are combined in a single solution. One of the oldest single-step approaches to trichrome staining is van Gieson's method, which stains muscle and cytoplasm yellow, and collagen red. Another is the Gömöri trichrome stain, which closely mimics Masson's trichrome. In "yellowsolve ...
As strong oxidants, they cross-link lipids mainly by reacting with unsaturated carbon-carbon bonds, and thereby both fix biological membranes in place in tissue samples and simultaneously stain them. [3] [4] The choice of negative stain in electron microscopy can be very important. An early study of plant viruses using negatively stained leaf ...
Hematoxylin and eosin stain (or haematoxylin and eosin stain or hematoxylin-eosin stain; often abbreviated as H&E stain or HE stain) is one of the principal tissue stains used in histology. [ 1 ] [ 2 ] [ 3 ] It is the most widely used stain in medical diagnosis [ 1 ] and is often the gold standard . [ 4 ]