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Pages in category "Peptide coupling reagents" The following 14 pages are in this category, out of 14 total. This list may not reflect recent changes. B. BOP reagent; D.
Coupling reagents (e.g. HBTU, HATU, or DIC) are employed to help form the peptide bond. The final deprotection is followed by a cleavage . The established method for the production of synthetic peptides in the lab is known as solid phase peptide synthesis (SPPS). [ 2 ]
HATU (Hexafluorophosphate Azabenzotriazole Tetramethyl Uronium) is a reagent used in peptide coupling chemistry to generate an active ester from a carboxylic acid. HATU is used along with Hünig's base (N,N-diisopropylethylamine), or triethylamine to form amide bonds.
1-Hydroxy-7-azabenzotriazole (HOAt) is a triazole used as a peptide coupling reagent. [2] It suppresses racemization that can otherwise occur during the reaction. [3] HOAt has a melting point of 213-216 °C. [4]
Common uses for this carbodiimide include peptide synthesis, protein crosslinking to nucleic acids, but also in the preparation of immunoconjugates. EDC is often used in combination with N-hydroxysuccinimide (NHS) for the immobilisation of large biomolecules. Recent work has also used EDC to assess the structure state of uracil nucleobases in RNA.
N,N ′-Diisopropylcarbodiimide is a carbodiimide used in peptide synthesis. [1] [2] As a liquid, it is easier to handle than the commonly used N,N ′-dicyclohexylcarbodiimide, a waxy solid. In addition, N,N ′-diisopropylurea, its byproduct in many chemical reactions, is soluble in most organic solvents, a property that facilitates work-up.