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HPLC has many applications in both laboratory and clinical science. It is a common technique used in pharmaceutical development, as it is a dependable way to obtain and ensure product purity. [59] While HPLC can produce extremely high quality (pure) products, it is not always the primary method used in the production of bulk drug materials. [60]
Ion suppression in LC-MS and LC-MS/MS refers to reduced detector response, or signal:noise as a manifested effect of competition for ionisation efficiency in the ionisation source, between the analyte(s) of interest and other endogenous or exogenous (e.g. plasticisers extracted from plastic tubes, [1] mobile phase additives) species which have not been removed from the sample matrix during ...
Silica gel particles are commonly used as a stationary phase in high-performance liquid chromatography (HPLC) for several reasons, [13] [14] including: High surface area: Silica gel particles have a high surface area, allowing direct interactions with solutes or after bonding of variety of ligands for versatile interactions with the sample molecules, leading to better separations.
The problem of having too high a concentration of salt in the sample can be solved by first running the solution through liquid chromatography to help purify the sample, but this method is time-consuming and results in the loss of some of the sample to be analyzed.
Rapidly, it was realized that the analysis of complex mixtures would require the development of a fully automated on-line coupling solution in LC–MS. [8] The key to the success and widespread adoption of LC–MS as a routine analytical tool lies in the interface and ion source between the liquid-based LC and the vacuum-base MS.
This is a list of unsolved problems in chemistry. Problems in chemistry are considered unsolved when an expert in the field considers it unsolved or when several experts in the field disagree about a solution to a problem.
Solvophobic theory attempts to explain interactions between polar solvents and non-polar solutes.In the pure solvent, there are relatively strong cohesive forces between the solvent molecules due to hydrogen bonding or other polar interactions.
Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.
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