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Alphacoronavirus chicagoense [1] ( also called Human coronavirus 229E and abbreviated HCoV-229E) is a species of coronavirus which infects humans and bats. [2] It is an enveloped , positive-sense , single-stranded RNA virus which enters its host cell by binding to the APN receptor . [ 3 ]
Alphacoronavirus amsterdamense [1] ( also called Human coronavirus NL63 abbreviated HCoV-NL63) is a species of coronavirus, specifically a Setracovirus from among the Alphacoronavirus genus. It was identified in late 2004 in patients in the Netherlands by Lia van der Hoek and Krzysztof Pyrc [2] using a novel virus discovery method VIDISCA. [3]
The extraction of RNA in molecular biology experiments is greatly complicated by the presence of ubiquitous and hardy RNases that degrade RNA samples. Certain RNases can be extremely hardy and inactivating them is difficult compared to neutralizing DNases. In addition to the cellular RNases that are released there are several RNases that are ...
It interacts with the viral proteins (M and N) [1] and ensures the selective packaging of viral RNA into virions. [2] This RNA element is conserved in Embecovirus (previously known as lineage A Betacoronavirus [3]), which includes mouse hepatitis virus (MHV), bovine coronavirus (BCoV), and human coronaviruses like HCoV-HKU1 and HCoV-OC43 ...
Human coronavirus OC43 [1] (HCoV-OC43) is a member of the species Betacoronavirus 1, which infects humans and cattle. [ 2 ] [ 3 ] The infecting coronavirus is an enveloped , positive-sense , single-stranded RNA virus that enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor . [ 4 ]
Betacoronavirus hongkonense [1] ( commonly called Human coronavirus HKU1 abbreviated as HCoV-HKU1) is a species of coronavirus in humans and animals. It causes an upper respiratory disease with symptoms of the common cold, but can advance to pneumonia and bronchiolitis. [2] It was first discovered in January 2004 from one man in Hong Kong. [3]
Although biological systems are incredibly diverse, RNA extraction techniques are broadly similar and involve mechanical disruption of cells or tissues, disruption of RNase with chaotropic salts, [44] disruption of macromolecules and nucleotide complexes, separation of RNA from undesired biomolecules including DNA, and concentration of the RNA ...
Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact tissue. [1] The historical precursor to spatial transcriptomics is in situ hybridization, [2] where the modernized omics terminology refers to the measurement of all the mRNA in a cell rather than select RNA targets.