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ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer. The amount of light produced is directly proportional to the amount of ATP present in the sample. ATP tests can be used to: Control biological treatment reactors; Guide biocide dosing programs
ATP is a molecule found only in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally occurring enzyme firefly luciferase using a luminometer. The amount of light produced is directly proportional to the ...
The ATP Assay Concept is a well-known technique for determining the viability of cells using the assessment of ATP and a method known as "firefly luciferase". [ 5 ] Sodium-potassium ratio: Another kind of assay practices the examination of the ratio of potassium to sodium in cells to serve as an index of viability.
Purine metabolites: Nucleotides (e.g., ATP) and nucleosides (e.g., adenosine) that have reached the extracellular space can also serve as danger signals by signaling through purinergic receptors. [30] ATP and adenosine are released in high concentrations after catastrophic disruption of the cell, as occurs in necrotic cell death. [31]
Unlike obligate anaerobes, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube. A facultative anaerobic organism is an organism that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation if oxygen is absent. [1] [2]
They gather mostly at the top because aerobic respiration generates more ATP than either fermentation or anaerobic respiration. 4: Microaerophiles need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube but not the very top.
ATP Binding Cassette or efflux transporters mediate the transport of substrates across cell membranes against a concentration gradient. ATP cleavage is tightly linked to substrate translocation, as the energy for the substrate translocation is derived from ATP hydrolysis. ATP hydrolysis yields inorganic phosphate (Pi), which can be measured by ...
ATP test doesn't really describe what the test is; The test is actually a living organism detection assay, which looks for ATP. I'm guessing ATP test is jargon. Is there a more descriptive name? Pdcook 01:56, 17 October 2009 (UTC) The name for the article is chosen based on an alternate testing method called 'Plate Count' or 'Culture test'.