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Genome (DNA) sequencing. Single-cell DNA genome sequencing involves isolating a single cell, amplifying the whole genome or region of interest, constructing sequencing libraries, and then applying next-generation DNA sequencing (for example Illumina, Ion Torrent). Single-cell DNA sequencing has been widely applied in mammalian systems to study ...
This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In the field of cellular biology, single-cell analysis and subcellular analysis [1] is the study of genomics, transcriptomics, proteomics, metabolomics and cell–cell interactions at the single cell level.
Single-cell transcriptomics. Single-cell transcriptomics examines the gene expression level of individual cells in a given population by simultaneously measuring the RNA concentration (conventionally only messenger RNA (mRNA)) of hundreds to thousands of genes. [1] Single-cell transcriptomics makes it possible to unravel heterogeneous cell ...
Transcriptome. The transcriptome is the set of all RNA transcripts, including coding and non-coding, in an individual or a population of cells. The term can also sometimes be used to refer to all RNAs, or just mRNA, depending on the particular experiment. The term transcriptome is a portmanteau of the words transcript and genome; it is ...
Single-cell DNA template strand sequencing, or Strand-seq, is a technique for the selective sequencing of a daughter cell's parental template strands. [1] This technique offers a wide variety of applications, including the identification of sister chromatid exchanges in the parental cell prior to segregation, the assessment of non-random segregation of sister chromatids, the identification of ...
List of single cell omics methods. A list of more than 100 different single cell sequencing (omics) methods have been published. [1] The large majority of methods are paired with short-read sequencing technologies, although some of them are compatible with long read sequencing.
G&T-seq (short for single cell genome and transcriptome sequencing) is a novel form of single cell sequencing technique allowing one to simultaneously obtain both transcriptomic and genomic data from single cells, allowing for direct comparison of gene expression data to its corresponding genomic data in the same cell...
An overview of the Hi-C workflow and its applications in research. Figure made using BioRender. Hi-C is a high-throughput genomic and epigenomic technique to capture chromatin conformation (3C). [1] In general, Hi-C is considered as a derivative of a series of chromosome conformation capture technologies, including but not limited to 3C ...