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In most cases the proton-motive force is generated by an electron transport chain which acts as a proton pump, using the Gibbs free energy of redox reactions to pump protons (hydrogen ions) out across the membrane, separating the charge across the membrane. In mitochondria, energy released by the electron transport chain is used to move protons ...
The proton motive force and ATP production can be maintained by intracellular acidosis. [88] Cytosolic protons that have accumulated with ATP hydrolysis and lactic acidosis can freely diffuse across the mitochondrial outer-membrane and acidify the inter-membrane space, hence directly contributing to the proton motive force and ATP production.
This transport chain produces a proton-motive force, pumping H + ions across the membrane and producing a concentration gradient that can be used to power ATP synthase during chemiosmosis. This pathway is known as cyclic photophosphorylation, and it produces neither O 2 nor NADPH.
The resulting proton gradient across the thylakoid membrane creates a proton-motive force, used by ATP synthase to form ATP. In cyclic photophosphorylation, cytochrome b 6 f uses electrons and energy from PSI to create more ATP and to stop the production of NADPH.
Bacteriorhodopsin is a light-driven H + ion transporter found in some haloarchaea, most notably Halobacterium salinarum (formerly known as syn. H. halobium).The proton-motive force generated by the protein is used by ATP synthase to generate adenosine triphosphate (ATP).
This change in both the pH and electrochemical potential gradient between the inside of the cell and the outside produces a proton-motive force, as the protons will want to naturally flow back into the area of low concentration and with a voltage closer to zero from their current situation of being in an area of high concentration of positively ...
The result is that the cell or mitochondrion expends energy to generate a proton-motive force, but the proton-motive force is dissipated before the ATP synthase can recapture this energy and use it to make ATP. Because the intracellular supply of protons is replenished, uncouplers actually stimulate cellular metabolism and oxygen consumption ...
The photon energy absorbed by Photosystem I also produces a proton-motive force that is used to generate ATP. PSI is composed of more than 110 cofactors , significantly more than Photosystem II . [ 3 ]