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Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
2. Atomic absorption spectrophotometer (AAS): Uses absorption of light by vaporized analyte atoms to determine concentrations of metals and metalloids. 3. Fluorescence spectrophotometer: Measures intensity of fluorescent light emitted from samples after excitation. Allows highly sensitive analysis of samples with native or induced fluorescence. 4.
The combination of high gain, low noise, high frequency response or, equivalently, ultra-fast response, and large area of collection has maintained photomultipliers an essential place in low light level spectroscopy, confocal microscopy, Raman spectroscopy, fluorescence spectroscopy, nuclear and particle physics, astronomy, medical diagnostics ...
Fluorescent proteins are used to tag components in cells so that they can be studied using fluorescence spectroscopy and fluorescence microscopy. mCherry absorbs light between 540 and 590 nm and emits light in the range of 550-650 nm.
Homogeneous, mix-and-read TR-FRET assays offer advantages over other biomolecular screening assays, such as fluorescence polarization (FP) or TRF assays. [3] In FP assays, background fluorescence due to library compounds is normally depolarized and background signal due to scattered light (e.g. precipitated compounds) is normally polarized.
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]
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