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To kill all spores in a clear liquid can take up to 22 hours with glutaraldehyde and even longer with formaldehyde. The presence of solid particles may lengthen the required period or render the treatment ineffective. Sterilization of blocks of tissue can take much longer, due to the time required for the fixative to penetrate.
The Tyndallization process is usually effective in practice. But it is not considered completely reliable — some spores may survive and later germinate and multiply. It is not often used today, but is used for sterilizing items that cannot withstand pressurized heating, such as plant seeds. [2] [3]
Moist heat sterilization processes sterilize using hot air that is heavily laden with water vapor, which plays the most important role in the sterilization. [1] [2] Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores, which can germinate shortly thereafter and resume growth ...
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Irradiated mail is mail that has been deliberately exposed to radiation, typically in an effort to disinfect it. The most notable instance of mail irradiation in the US occurred in response to the 2001 anthrax attacks; the level of radiation chosen to kill anthrax spores was so high that it often changed the physical appearance of the mail.
Soil steam sterilization (soil steaming) is a farming technique that sterilizes soil with steam in open fields or greenhouses. Pests of plant cultures such as weeds, bacteria, fungi and viruses are killed through induced hot steam which causes vital cellular proteins to unfold .
To prove sterility, biological indicators are used. Biological indicators contain spores of a heat-resistant bacterium, Geobacillus stearothermophilus. If the autoclave does not reach the right temperature, the spores will germinate when incubated and their metabolism will change the color of a pH-sensitive chemical.
Ultraviolet germicidal irradiation (UVGI) is a disinfection technique employing ultraviolet (UV) light, particularly UV-C (180–280 nm), to kill or inactivate microorganisms. UVGI primarily inactivates microbes by damaging their genetic material, thereby inhibiting their capacity to carry out vital functions. [1]