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Making a standard solution requires great attention to detail to avoid introducing any risk of contamination that could diminish the accuracy of the concentration. For this reason, glassware with high degree of precision such as a volumetric flask, volumetric pipette, micropipettes, and automatic pipettes are used in the preparation steps. The ...
Sample preparation for mass spectrometry is used for the optimization of a sample for analysis in a mass spectrometer (MS). Each ionization method has certain factors that must be considered for that method to be successful, such as volume, concentration, sample phase, and composition of the analyte solution. Quite possibly the most important ...
Plasmid preparation can be divided into five main categories based on the scale of the preparation: minipreparation, midipreparation, maxipreparation, megapreparation, and gigapreparation. The choice of which method to use will depend on the amount of plasmid DNA required, as well as the specific application for which it will be used. [18] [19]
In analytical chemistry, sample preparation (working-up) refers to the ways in which a sample is treated prior to its analyses. Preparation is a very important step in most analytical techniques, because the techniques are often not responsive to the analyte in its in-situ form, or the results are distorted by interfering species .
Carrying iodine for water purification is an imperfect but lightweight solution for those in need of field purification of drinking water. Kits are available in camping stores that include an iodine pill and a second pill (vitamin C or ascorbic acid) that will remove the iodine taste from the water after it has been disinfected. The addition of ...
To apply this method, analysts prepare multiple solutions containing equal amounts of unknown and spike them with varying concentrations of the analyte. The amount of unknown and the total volume are the same across the standards and the only difference between the standards is the amount of analyte spiked.
Prepare 800 mL of distilled water in a suitable container. Add 8 g of NaCl to the solution. Add 400 mg of KCl to the solution. Add 140 mg of CaCl 2 to the solution. Add 100 mg of MgSO 4-7H 2 O to the solution. Add 100 mg of MgCl 2-6H 2 O to the solution. Add 60 mg of Na 2 HPO 4-2H 2 O to the solution. Add 60 mg of KH 2 PO 4 to the solution.
To create the solution, 11.6 g NaCl is placed in a volumetric flask, dissolved in some water, then followed by the addition of more water until the total volume reaches 100 mL. The density of water is approximately 1000 g/L and its molar mass is 18.02 g/mol (or 1/18.02 = 0.055 mol/g). Therefore, the molar concentration of water is