Ads
related to: how to use pipette correctly
Search results
Results From The WOW.Com Content Network
For example, a motorized pipette controller can aid liquid aspiration or dispensing using volumetric pipettes or graduated pipettes; [13] a tablet can interact in real-time with the pipette and guide a user through a protocol; [14] and a pipette station can help to control the pipette tip immersion depth and improve ergonomics. [15]
Then use a propipetter, a pipette bulb, or rubber bulb, to draw the liquid into the pipette. The effective way to control the volume of the solution is to use one's forefinger. [ 2 ] After getting the desired volume, the solution can be released into another vessel by lifting the finger.
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop (#3). Withdraw the tip from the liquid. Some liquid will remain inside the tip. The liquid remaining in the tip can be dispensed back into the original solution (#4) or thrown away. Release the pipette thumb to the ready ...
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop. After about one second, keep on pressing pipette knob to the second stop. This action will completely empty the tip from the liquid. Afterwards withdraw the tip from the liquid and release the pipette to the ready position.
Generally, a pipette has optimal accuracy from 35-100% of its nominal volume, and should not be used below 10% of that volume. [5] For example, using a 1,000 μl pipette for 50 μl of liquid is not ideal, and using a 100 μl pipette will give better results. [6] Other factors like tip angle and immersion depth may also impact accuracy ...
A Mohr pipette, also known as a graduated pipette, is a type of pipette used to measure the volume of the liquid dispensed, although not as accurately as a volumetric pipette. [1] These use a series of marked lines (as on a graduated cylinder) to indicate the different volumes. [ 2 ]