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In the original Pearson FASTA format, one or more comments, distinguished by a semi-colon at the beginning of the line, may occur after the header. Some databases and bioinformatics applications do not recognize these comments and follow the NCBI FASTA specification. An example of a multiple sequence FASTA file follows:
The FAST4 format was invented as a derivative of the FASTQ format where each of the 4 bases (A,C,G,T) had separate probabilities stored. It was part of the Swift basecaller, an open source package for primary data analysis on next-gen sequence data "from images to basecalls". The FAST5 format was invented as an extension of the FAST4 format.
UniProt is a freely accessible database of protein sequence and functional information, many entries being derived from genome sequencing projects.It contains a large amount of information about the biological function of proteins derived from the research literature.
HTML is the default output format for NCBI's web-page. Results for NCBI-BLAST are presented in graphical format with all the hits found, a table with sequence identifiers for the hits having scoring related data, along with the alignments for the sequence of interest and the hits received with analogous BLAST scores for these. [8]
The extensible NEXUS file format is widely used in phylogenetics, evolutionary biology, and bioinformatics.It stores information about taxa, morphological character states, DNA and protein sequence alignments, distances, and phylogenetic trees. [1]
FASTA is a DNA and protein sequence alignment software package first described by David J. Lipman and William R. Pearson in 1985. [1] Its legacy is the FASTA format which is now ubiquitous in bioinformatics .
The output is the predicted peptide sequences in the FASTA format, and a definition line that includes the query ID, the translation reading frame and the nucleotide positions where the coding region begins and ends. OrfPredictor facilitates the annotation of EST-derived sequences, particularly, for large-scale EST projects.
Aligned sequences will replace unaligned ones in the main section of the Alignment Editor. To perform further analysis in MEGA, it is advisable to save the alignment session in either MEGA or FASTA format. [5] Trace Data File Viewer/Editor ― The Trace Data File Viewer/Editor has many functionalities in the following three menus. All the ...