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Eukaryotic cells are some 10,000 times larger than prokaryotic cells by volume, have their DNA organised in a nucleus, and contain membrane-bound organelles. The division between prokaryotes and eukaryotes has been considered the most important distinction or difference among organisms.
The DNA of a prokaryotic cell consists of a single circular chromosome that is in direct contact with the cytoplasm. The nuclear region in the cytoplasm is called the nucleoid. Most prokaryotes are the smallest of all organisms, ranging from 0.5 to 2.0 μm in diameter. [1] [page needed] A prokaryotic cell has three regions:
Although prokaryotic organisms do not possess a membrane-bound nucleus like eukaryotes, they do contain a nucleoid region in which the main chromosome is found. Extrachromosomal DNA exists in prokaryotes outside the nucleoid region as circular or linear plasmids.
Most prokaryote chromosomes contain a circular DNA molecule. This has the major advantage of having no free ends to the DNA. By contrast, most eukaryotes have linear DNA requiring elaborate mechanisms to maintain the stability of the telomeres and replicate the DNA.
The nucleoid (meaning nucleus-like) is an irregularly shaped region within the prokaryotic cell that contains all or most of the genetic material. [1] [2] [3] The chromosome of a typical prokaryote is circular, and its length is very large compared to the cell dimensions, so it needs to be compacted in order to fit.
Genomic DNA is tightly and orderly packed in the process called DNA condensation, to fit the small available volumes of the cell. In eukaryotes, DNA is located in the cell nucleus, with small amounts in mitochondria and chloroplasts. In prokaryotes, the DNA is held within an irregularly shaped body in the cytoplasm called the nucleoid. [97]
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Prokaryotic cells have entirely different structures for organizing their DNA (the prokaryotic chromosome equivalent is called a genophore and is localized within the nucleoid region). The overall structure of the chromatin network further depends on the stage of the cell cycle.