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NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR ...
The software designs real-time PCR primers and probes for complex differential gene expression and allele discrimination assays. Beacon Designer has been used in workshops and conferences related to real-time PCR study as a reference software.
As with Adobe Acrobat, Nitro PDF Pro's reader is free; but unlike Adobe's free reader, Nitro's free reader allows PDF creation (via a virtual printer driver, or by specifying a filename in the reader's interface, or by drag-'n-drop of a file to Nitro PDF Reader's Windows desktop icon); Ghostscript not needed.
The last 10-12 bases at the 3' end of a primer are sensitive to initiation of polymerase extension and general primer stability on the template binding site. The effect of a single mismatch at these last 10 bases at the 3' end of the primer depends on its position and local structure, reducing the primer binding, selectivity, and PCR efficiency.
OLIGO Primer Analysis Software is a software for DNA primer design. [ 1 ] [ 2 ] The first paper describing this software was published in 1989. [ 3 ] The program is a real time PCR primer and probe search and analysis tool.
Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...
The free NCBI tool Primer-BLAST integrates primer design and BLAST search into one application, [6] as do commercial software products such as ePrime and Beacon Designer. Computer simulations of theoretical PCR results ( Electronic PCR ) may be performed to assist in primer design by giving melting and annealing temperatures, etc. [ 7 ]
This is a category of articles relating to free software for making or viewing Portable Document Format (PDF) documents. That is, software which can be freely used, copied, studied, modified, and redistributed by everyone that obtains a copy. Typically, this means software which is distributed with a free software license, and whose source code ...