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  2. Ion chromatography - Wikipedia

    en.wikipedia.org/wiki/Ion_chromatography

    Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger. [1] It works on almost any kind of charged molecule —including small inorganic anions, [ 2 ] large proteins , [ 3 ] small nucleotides , [ 4 ] and amino acids .

  3. Protein purification - Wikipedia

    en.wikipedia.org/wiki/Protein_purification

    Ion exchange chromatography is a very powerful tool for use in protein purification and is frequently used in both analytical and preparative separations. It is especially useful when purifying nucleic-acid binding proteins, where separation of the protein from the bound nucleic acid is required to obtain a pure sample devoid of nucleic acids ...

  4. Chromatography in blood processing - Wikipedia

    en.wikipedia.org/wiki/Chromatography_in_blood...

    ion exchange on DEAE Sepharose is run to bind the albumin to the column, Albumin is eluted with a sodium acetate buffer, and; Final polishing with gel filtration. The end result is a highly pure and safe batch of albumin that is 100% non-pyrogenic, sterile, and free of active HIV virus. The product purity is greater than 98% and the protein ...

  5. Mixed-mode chromatography - Wikipedia

    en.wikipedia.org/wiki/Mixed-mode_chromatography

    In 1986, Regnier’s group synthesized a stationary phase that had characteristics of anion exchange chromatography (AEX) and hydrophobic interaction chromatography (HIC) on protein separation. [8] In 1998, a new form of MMC, hydrophobic charge induction chromatography (HCIC), was proposed by Burton and Harding.

  6. Ion Exchange Chromatography - Wikipedia

    en.wikipedia.org/?title=Ion_Exchange...

    Ion Exchange Chromatography. Add languages ... Upload file; Special pages; Permanent link; Page information; Cite this page; Get shortened URL; Download QR code ...

  7. Hydrophilic interaction chromatography - Wikipedia

    en.wikipedia.org/wiki/Hydrophilic_interaction...

    Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography.

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