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In science, ex vivo refers to experimentation or measurements done in or on tissue from an organism in an external environment with minimal alteration of natural conditions. [ 2 ] A primary advantage of using ex vivo tissues is the ability to perform tests or measurements that would otherwise not be possible or ethical in living subjects.
Ex vivo reconstruction, short for ex vivo renal artery reconstruction and autotransplantation, is a technique mainly used for complex disease involving multiple ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Once life support has been withdrawn, there is a 2-5 minute waiting period to ensure that the potential donor's heart does not start beating again spontaneously. [4] After this waiting period, the organ procurement surgery begins as quickly as possible to minimize time that the organs are not being perfused with blood.
This ex vivo model requires a highly maintained environment in order to recreate original cellular conditions. The composition of extracellular matrix, for example, must be precisely similar to that of in vivo conditions in order to induce naturally observed behaviors of cells. The growth medium also must be considered, as different solutions ...
Resection of liver can induce the proliferation of the remaining hepatocytes until the lost mass is restored, where the intensity of the liver's response is directly proportional to the mass resected. For almost 80 years surgical resection of the liver in rodents has been a very useful model to the study of cell proliferation. [37] [38]
Ex vivo lung perfusion, EVLP, is a form of machine perfusion aimed at sustaining the active aerobic cellular metabolism of donor lungs outside the donor's body prior to lung transplantation. This medical preservation technique typically occurs within a specialised machine engineered to mimic the conditions of the natural circulatory system .
Cell culture in a small Petri dish Epithelial cells in culture, stained for keratin (red) and DNA (green). Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment.