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[2] A primary advantage of using ex vivo tissues is the ability to perform tests or measurements that would otherwise not be possible or ethical in living subjects. Tissues may be removed in many ways, including in part, as whole organs, or as larger organ systems. [citation needed] Examples of ex vivo specimen use include: [citation needed]
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Once life support has been withdrawn, there is a 2-5 minute waiting period to ensure that the potential donor's heart does not start beating again spontaneously. [4] After this waiting period, the organ procurement surgery begins as quickly as possible to minimize time that the organs are not being perfused with blood.
Cell culture in a small Petri dish Epithelial cells in culture, stained for keratin (red) and DNA (green). Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment.
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism.
By 2009, via the use of materials, a max induced regeneration could be achieved inside a 1 cm tissue rupture. [2] Bridging the wound, the material allowed cells to cross the wound gap; the material then degraded. This technology was first used inside a broken urethra in 1996. [2] [3] In 2012, using materials, a full urethra was restored in vivo ...
This glossary of biology terms is a list of definitions of fundamental terms and concepts used in biology, the study of life and of living organisms.It is intended as introductory material for novices; for more specific and technical definitions from sub-disciplines and related fields, see Glossary of cell biology, Glossary of genetics, Glossary of evolutionary biology, Glossary of ecology ...
A skinpatch test has been designed and is used in Canada to measure development of rashes, inflammation, swelling or abnormal tissue growth on human volunteers. [25] Unlike corrosives, substances defined as irritants cause only reversible skin damage. Another approach has been the development of test methods that use cultured human cells.